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11/6/2025

Q&A: How to Test for Hydrogen Sulfide SIBO?

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This video explores the two main ways to test for Hydrogen Sulfide SIBO (Small Intestinal Bacterial Overgrowth), more recently known as ISO (Intestinal Sulfide Overproduction), explaining both newer direct methods and older indirect approaches of testing. 
  1. Trio-Smart test directly tests hydrogen sulfide gas. This test was developed by Dr. Mark Pimentel and is obtained from Gemelli lab.
  2. 3-hour Lactulose SIBO test (testing hydrogen and methane). When there is a flat line pattern, it indicates hydrogen sulfide, but misses many cases.

Why Hydrogen Sulfide SIBO Testing Matters:
Because it was harder to test for this gas directly in the past, many cases of Hydrogen Sulfide SIBO went undiagnosed. People might have had negative results for hydrogen and methane SIBO tests, yet still had SIBO. Now, with better testing methods, doctors can identify this specific type of SIBO more accurately. This leads to more targeted treatment plans for the type of SIBO.

Conclusion
The new trio-smart breath test now offers a direct way to measure hydrogen sulfide along with hydrogen and methane. This helps find more cases of SIBO. Before this, the flatline pattern from a standard breath test offered an indirect testing method, but it often missed many cases.

Products mentioned:
~ Trio-smart SIBO test

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  • HOME
    • HOME
  • About SIBO
    • WHAT IS SIBO?
    • SIBO Symptoms
    • Associated Diseases
    • What Causes SIBO?
    • SIBO & IBS
  • SIBO TESTS
    • SIBO Tests
  • TREATMENT
    • How To Treat SIBO
    • Symptom Relief
    • What to Avoid
    • Antibiotics
    • Herbal Antibiotics
    • Elemental Diet
    • SIBO Diet
    • Prevention of Relapse
    • Probiotics
  • SIBO COURSES
    • SIBO Courses
  • SIBO SUPPORT
    • SIBO Support
  • RESOURCES
    • Discounts
    • BLOG
    • SIBO Podcasts
    • SIBO Studies >
      • SIBO Studies: 2025
      • SIBO Studies: 2024
      • SIBO Studies: 2023
      • SIBO Studies: 2022
      • SIBO Studies: 2021
      • SIBO Studies: 2020
      • SIBO Studies: 2019
      • SIBO Studies: 2018
      • SIBO Studies: 2017
      • SIBO Studies: 2016
      • SIBO Studies: 2015
      • SIBO Studies: 2014
      • SIBO Studies: 2013
      • SIBO Studies: 2012
      • SIBO Studies: 2011
      • SIBO Studies: 2009
      • SIBO Studies: 1990's
      • SIBO Studies: 1980's
    • SIBO Books
    • SIBO Recipes & Cookbooks
    • MMC Videos
    • Test Directory
    • Finding a SIBO Doctor
  • CONTACT
    • Contact
    • About Dr. Siebecker
    • Online Consultations
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